Electron Transfer Proteins of Cytochrome P450 Systems

نویسندگان

  • Israel Hanukoglu
  • ISRAEL HANUKOGLU
چکیده

I . INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . , 2 9 SEQUENCE AND STRUCTURAL RELATIONSHIPS . . . . . . . . . . . . , 32 A. Adrenodoxin Reductase and Putidaredoxin Reductase . . . . . . . . . . . 32 H. Adrenodoxin and Putidaredoxin . , . . . . . . . . . . . . . . . . . . . . . 36 C. P450Reductase. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 8 I l l . LEVELS OF EXPRESSION . . . . . . . . . . . . . . . . . . . . . . . . . . , 4 0 A. Mitochondria1 and Bacterial P450cam Systems . . . . . . . . . . . . . . . 40 B. Microsomal Systems . . . . . . . . . . . . . . . . . . . , . . . . . . . . . 4 1

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Redundancy or flexibility: molecular diversity of the electron transfer components for P450 monooxygenases in higher plants.

Specific metabolic roles of P450-dependent monooxygenase systems are determined by enzymatic properties and substrate specificity of P450s, the terminal enzymes of the electron transfer chain. On the other hand, molecular diversity has also been reported for NADPH-cytochrome P450 reductase, cytochrome b5, and cytochrome b5 reductase in plants. Several lines of evidence indicate that the electro...

متن کامل

Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure.

Cytochrome P450 (P450) is an attractive oxygenase due to the diverse catalytic reactions and the broad substrate specificity. Class I P450s require an excess concentration (more than 10 times) of iron-sulfur proteins, which transfer electrons to P450s, to attain the maximum catalytic activity and this requirement is a critical bottleneck for practical applications. Here, we show a site-specific...

متن کامل

Cytochrome P450 proteins and potential utilization in biodegradation.

The cytochrome P450 enzymes are major catalysts involved in the oxidations of xenobiotic chemicals in microorganisms as well as higher animals and plants. Because of their functional roles, they offer potential in biodegradation technology. A number of microbial P450s have already been characterized and offer advantages in terms of their high catalytic rates and facile expression in microorgani...

متن کامل

Structural domains of P450-containing monooxygenase systems.

All known P450-containing monooxygenase systems share common structural and functional domain architecture. Apart from P450 itself, these systems can comprise several fundamentally different protein components or domains, all of which are shared by other multicomponent/multidomain enzyme systems with various functions: FAD flavoprotein or domain, FMN domain, Fe2S2 ferredoxin, Fe3S4 ferredoxin, ...

متن کامل

Electron transfer in human cytochrome P450 reductase.

Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiom...

متن کامل

Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2008